Product Name :
Phospho-STAT5 (Y694) Recombinant Rabbit Monoclonal Antibody [SC05-31]
Predicted band size :
91 kDa
Observed band size :
91 kDa
Synonyms:
MGF antibody Signal transducer and activator of transcription 5A antibody Signal Transducer and Activator of Transcription 5B antibody STA5A_HUMAN antibody STAT 5A antibody STAT 5B antibody STAT5 antibody STAT5A antibody STAT5B antibody Transcription factor STAT5A antibody 展开
Function :
Stat5 (Signal Transducers and Activators of Transcription 5) is important in regulating T cell functions involving the receptors for Interleukin-2 (IL-2). IL-2 stimulates the rapid phosphorylation of both serine and tyrosine residues of Stat5a and Stat5b in human T lymphocytes and in several IL-2-responsive lymphocytic cell lines. IL-2 differentially induces serine phosphorylation of Stat5a and Stat5b on Ser726 and Ser731, respectively. Stat5b is preferentially phosphorylated and displays more protracted serine phosphorylation kinetics than Stat5a. Both the acid-rich region and the COOH terminus of IL-2Rb can independently mediate IL-2-induced Stat 5a/b serine phosphorylation, suggesting that Stat5a/b serine phosphorylation occurs at a postreceptor level. Stat5a is phosphorylated on Tyr694 in a prolactin-sensitive manner, whereas serine phosphorylation is constitutive. Activation of Stat5 by IL-2 may help govern the biological effects of IL-2 during the immune response.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Tyr694 of human STAT5A.
Species reactivity:
Human, Rat, Mouse
Verify application:
WB, IHC-P, IF-Cell
Coupling :
unconjugated
Clone number:
SC05-31
RRID:
AB_3069933
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.1190310-00-9 supplier Aliquot store at -20℃ or -80℃.19393-83-0 structure Avoid repeated freeze / thaw cycles.PMID:33403573
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 91 kDa
Positive control :
PC-12 treated with 1mM Sodium orthovanadate for 30 minutes cell lysate, PC-12 cells treated with 1mM Sodium orthovanadate for 30 minutes, Hela cells treated with 100ng/mL IFN alpha for 60 minutes, human breast carcinoma tissue.