Product Name :
PUF60 Rabbit Polyclonal Antibody
Predicted band size :
Observed band size :
Synonyms:
60 kDa poly(U)-binding-splicing factor antibody FBP-interacting repressor antibody FIR antibody FLJ31379 antibody FUSE-binding protein-interacting repressor antibody Poly U binding splicing factor 60KDa antibody Poly(U) binding splicing factor 60 antibody Poly(U)-binding-splicing factor PUF60 antibody poly-U binding splicing factor PUF60 antibody PUF60 antibody 展开
Function :
DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3′-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3′-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2.2369772-11-0 Chemscene Binds to Y5 RNA in association with TROVE2.2-Bromo-4-chloro-6-methoxypyridine web Binds to poly(U) RNA.PMID:33751641
Antibody Type:
Rabbit Polyclonal Antibody
Immunogen :
KLH conjugated synthetic peptide derived from human PUF60 1-100/559
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, ELISA, IHC-P, FC, IF
Coupling :
unconjugated
Clone number:
RRID:
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage buffer solution:
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
60 KDa
Positive control :
Isoform 2 is expressed in colonic epithelium and colorectal epithelium cancer (at protein level). Isoform 6 is expressed in colorectal epithelial cancer but below detection level in colonic epithelium. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.